0 citations

The Large-Scale Isolation of Bryostatin 1 from Bugula neritina Following Current Good Manufacturing Practices

Journal of Natural Products1991Vol. 54(5), pp. 1265–1270

Citations Over TimeTop 19% of 1991 papers

Daniel Schaufelberger, Mary P. Koleck, John A. Beutler, Argiro Vatakis, A. Belinda Alvarado, Pamela Andrews, L. V. Marzo, Gary M. Muschik, John Roach, John T. Ross, William B. Lebherz, M. Reeves, Robert M. Eberwein, Lori L. Rodgers, Robert P. Testerman, Kenneth M. Snader, Salvatore Forenza

Abstract

A novel process was designed for the large-scale isolation of bryostatin 1 from the bryozoan Bugula neritina L. in order to obtain multigram quantities of highly pure material for formulation studies, preclinical toxicology, and clinical trials in cancer patients. Multigram quantities of bryostatin 1 were obtained from a collection of approximately 10,000 gallons of wet animal. A phorbol dibutyrate (PDBu) receptor binding assay and hplc with photodiode array detection were used for the design, validation, and control of the isolation process.

Related Papers

Susquehanna Chorale Spring Concert "Roots and Wings"(2017)
→ DETERMINING QUALITY REQUIREMENTS AT THE UNIVERSITIES TO IMPROVE THE QUALITY OF EDUCATION(2018)
Коммуникaтивно- прaгмaтический aнaлиз дипломaтических бумaг (нa основе вербaльных нот)(2018)
→ ФОРМИРОВAНИЕ ГОТОВНОСТИ БУДУЩИХ ПЕДAГОГОВ К ОРГAНИЗAЦИИ РAБОТЫ ПО РAЗВИТИЮ ВAЛЕОЛОГИЧЕСКОЙ КУЛЬТУРЫ ШКОЛЬНИКОВ(2023)