Synthesis and Characterization of a New Red-Emitting Ca2+ Indicator, Calcium Ruby
Organic Letters2007Vol. 9(14), pp. 2629–2632
Citations Over TimeTop 25% of 2007 papers
Stéphane Gaillard, Aleksey V. Yakovlev, Camilla Luccardini, Martin Oheim, Anne Feltz, Jean‐Maurice Mallet
Abstract
Calcium Ruby m-Cl (X = H, Y = Cl) is a visible-light excited red-emitting calcium concentration ([Ca2+]) indicator dye (579/598 nm peak excitation/emission) with a side arm for conjugation via EDC or click chemistry. Its large molar extinction and high quantum yield rank it among the brightest long-wavelength Ca2+ indicators. Calcium Ruby is a promising alternative to existing dyes for imaging [Ca2+] in multicolor fluorescence applications or in the presence of yellow-green cellular autofluorescence.
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