Synthesis and RNA Binding Selectivity of Oligonucleotides Modified with Five-Atom Thioacetamido Nucleic Acid Backbone Structures

Citations Over Time

Abstract

Convenient chemical synthesis and incorporation of dithymidine and thymidine-cytidine dimer blocks connected with a five-atom amide linker N3'-CO-CH2-S-CH2 into oligonucleotides (ONs) are reported. The UV-Tm experiments for binding affinities of these mixed backbone ONs with complementary DNA and RNA sequences revealed important results such as significantly higher RNA-binding selectivity as compared with complementary DNA. NMR studies of the dimer blocks suggested a marginal increase in the N-type sugar conformations over that of the native DNA.

Related Papers

• → Enhancement by Cytidine of Membrane Phospholipid Synthesis(1992)56 cited
• → Metabolism of 4- N -Hydroxy-Cytidine in Escherichia coli(1971)13 cited
• → Craniodental Affinities of Southeast Asia's “Negritos” and the Concordance with Their Genetic Affinities(2013)5 cited
• → The effect of pH on the adsorption and the association of cytidine, cytidine-5′-monophosphate and cytidine-5′-diphosphate at the charged interface(1986)15 cited
• → The influence of cytidine on the endogenous pool of CDP-choline, CDP-ethanolamine, and CMP of the rat brain(1984)6 cited