Synthesis of 5-Hydroxymethyl-, 5-Formyl-, and 5-Carboxycytidine-triphosphates and Their Incorporation into Oligonucleotides by Polymerase Chain Reaction
Organic Letters2013Vol. 15(2), pp. 366–369
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Barbara Steigenberger, Stefan Schießer, Benjamin Hackner, Caterina Brandmayr, Silvia K. Laube, Jessica Steinbacher, Toni Pfaffeneder, Thomas Carell
Abstract
The synthesis of the triphosphates of 5-hydroxymethyl-, 5-formyl-, and 5-carboxycytidine and the incorporation of these building blocks into long DNA fragments using the polymerase chain reaction (PCR) are reported. In this way DNA fragments containing multiple hmC, fC, and caC nucleobases are readily accessible.
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