Selective Detection of 5-Formyl-2′-deoxycytidine in DNA Using a Fluorogenic Hydroxylamine Reagent
Organic Letters2013Vol. 15(13), pp. 3266–3269
Citations Over TimeTop 13% of 2013 papers
Pu Guo, Shengyong Yan, Jianlin Hu, Xiwen Xing, Changcheng Wang, Xiaowei Xu, Xiaoyu Qiu, Wen Ma, Chunjiang Lu, Xiaocheng Weng, Xiang Zhou
Abstract
Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.
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