Synthesis of New Fluorogenic Cyanine Dyes and Incorporation into RNA Fluoromodules
Organic Letters2008Vol. 10(8), pp. 1561–1564
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T Constantin, Gloria L. Silva, Kelly L. Robertson, Tamara P. Hamilton, Kaitlin Fague, Alan S. Waggoner, Bruce A. Armitage
Abstract
A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K(d) = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels.