Biocatalytic Oxidation of 2-Methylquinoxaline to 2-Quinoxalinecarboxylic Acid
Citations Over TimeTop 22% of 2002 papers
Abstract
A microbial process using the fungus Absidia repens ATCC 14849 is described for the oxidation of 2-methylquinoxaline to 2-quinoxalinecarboxylic acid. A campaign consisting of three 14000-L runs produced 20.5 kg of the acid with a 28% overall yield. The bioconversion gave a lower yield compared with a three step chemical synthesis (35%), but was carried out in one pot, and avoided safety issues with a di-N-oxide intermediate. Although successfully scaled to produce kilograms of 2-quinoxalinecarboxylic acid for synthesis of a drug candidate, the A. repens bioconversion is unsuitable for further scale-up due to low product concentration (∼1 g/L). A second microbial process using Pseudomonas putida ATCC 33015 is also described for the oxidation of 2-methylquinoxaline. The P. putida bioconversion gave an 86% in situ yield at 8-L scale and yielded a product concentration approximately 10-fold greater than that of the A. repens bioconversion.
Related Papers
- → Biotransformation of tabersonine in cell suspension cultures of Catharanthus roseus(1992)20 cited
- → Biotransformation of 2-acetylthiophene by micromycetes(1991)6 cited
- DEVELOPMENT OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF PRODUCTS ACCUMULATED DURING CHOLESTEROL BIOCONVERSION BY PSEUDOMONAS PUTIDA MTCC 1259(2011)
- Production Of L-Phenyl Acetyl Carbinol Through Biotransformation Of Benzaldehyde By Free And Immobilized Yeast Cells(2007)
- → Biological properties of risospheric bacteria pseudomonas putida and their biologically active metabolites in regulation of plant microbial systems(2019)