Direct MS/MS Analysis of Proteins Blotted on Membranes by a Matrix-Assisted Laser Desorption/Ionization-Quadrupole Ion Trap-Time-of-Flight Tandem Mass Spectrometer

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Abstract

We constructed a system for the microscale identification of membrane-blotted proteins by proteolytic digestion using an instrument developed with piezoelectric chemical inkjet technology and MS/MS analyses of the resulting peptides with a matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight tandem mass spectrometer (MALDI-QIT-TOF MS). Using this system, bovine serum albumin was clearly identified at levels less than 100 fmol, and proteins from an Escherichia coli extract were also identified by an MS/MS ion search.

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