Effects of Modified Digestion Schemes on the Identification of Proteins from Complex Mixtures
Journal of Proteome Research2006Vol. 5(3), pp. 695–700
Citations Over TimeTop 10% of 2006 papers
Abstract
In shotgun proteomics, a complex protein mixture is digested to peptides, separated, and identified by microcapillary liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). In this technology, complete protein digestion is often assumed. We show that, to the contrary, modifications to a standard digestion protocol demonstrate large, reproducible improvements in protein identification, a result consistent with digestion being a limiting factor in the efficiency of protein identification.
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