Using Annotated Peptide Mass Spectrum Libraries for Protein Identification

Citations Over TimeTop 1% of 2006 papers

Abstract

A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. This system was based on the annotated spectra currently available in the Global Proteome Machine Database (GPMDB). The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence modifications, and parent ion charge. The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, resulting in a peptide sequence identification based on scoring the similarity of the experimental spectrum with the contents of the library. A software implementation that performs this type of library search was constructed and successfully used to obtain sequence identifications. The annotated tandem mass spectrum libraries for the Homo sapiens, Mus musculus, and Saccharomyces cerevisiae proteomes and search software were made available for download and use by other groups.

Related Papers

• → A high-stringency blueprint of the human proteome(2020)215 cited
• → The human eye proteome project(2013)9 cited
• → Indian proteomics efforts and human proteome project(2015)5 cited
• → Human Proteome Project and Current Bioinformatics Status in Disease Diagnosis and Treatment(2018)1 cited
• → HICL table can manipulate all proteins in human complete proteome(2016)