Metabonomic Studies on the Physiological Effects of Acute and Chronic Psychological Stress in Sprague−Dawley Rats

Citations Over TimeTop 17% of 2007 papers

Abstract

The biochemical effects of acute and chronic psychological stress have been investigated in male Sprague-Dawley rats using a combination of 1H NMR spectral analysis of plasma and conventional hematological analyses. Animals were subjected to 35 consecutive days of 6-h sessions of stress, and following a 9 day break, were stressed for a further 6-h period. Plasma samples were collected at 0, 1, 3, and 6 h on days 1, 9, 21, 35, and 44, measured using 600 MHz 1H NMR spectroscopy, and analyzed by Principal Components Analysis. Time-dependent biochemical effects of psychological stress on a range of endogenous metabolites were evident and were correlated with the intensity of the stress response as defined by corticosterone and hematological parameters. Following acute stress, increases in the levels of glucose and ketone bodies, and decreases in the levels of acetate, alanine, isoleucine, lactate, leucine, valine, and lipoproteins, were observed. Chronic stress-induced increases in plasma levels of alanine, lactate (day 9), and leucine, valine, and choline (day 44) and decreases in acetate (day 9) and lipoprotein concentrations were observed. Positive correlations between plasma corticosterone level and glucose and glycerol, and between plasma lipoprotein concentrations and hemoglobin levels, were established using Projection to Latent Structures (PLS) analysis. This study indicates the potential of using NMR-based metabonomic strategies for the characterization of endogenous metabolic perturbations induced by psychological stressors and lifestyle choices.

Related Papers

• → Role of the adrenal cortex in chronic stress-induced inhibition of prolactin secretion in male rats(1989)28 cited
• → Chronic Iodine Deprivation Attenuates Stress‐Induced and Diurnal Variation in Corticosterone Secretion in Female Wistar Rats(2000)23 cited
• → CHOLINERGIC INFLUENCES ON HYPOTHALAMIC-PITUITARY-ADRENOCORTICAL ACTIVITY OF STRESSED RATS: AN APPROACH UTILIZING CHOLINE DEFICIENT DIETS(1978)12 cited
• → Administration of valine, leucine, and isoleucine improved plasma cholesterol and mitigated the preatherosclerotic lesions in rats fed with hypercholesterolemic diet(2018)5 cited
• → Postnatal Maturation Patterns of Serum Corticosterone and Growth Hormone in Rats: Effect of Chronic Thyroxine Administration(1979)30 cited