NopM and NopD Are Rhizobial Nodulation Outer Proteins: Identification Using LC-MALDI and LC-ESI with a Monolithic Capillary Column
Journal of Proteome Research2007Vol. 6(3), pp. 1029–1037
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João Rodrigues, Francisco Javier López‐Baena, Francisco Javier Ollero, José‐María Vinardell, M. Rosario Espuny, Ramón A. Bellogín, José E. Ruiz‐Sainz, Jerry R. Thomas, David Sumpton, James R. Ault, Jane Thomas‐Oates
Abstract
We have explored the potential of commercial polystyrene-divinylbenzene monolithic capillary nanoLC-MS/MS for identifying Sinorhizobium fredii HH103 nodulation outer proteins. Monolithic nanoLC with off-line MALDI-TOF/TOF and on-line ESI-q-oTOF is fast and robust, generating complementary data and offering high-confidence protein identifications from gel bands too weak for successful analysis using traditional approaches. This has allowed identification of two proteins not previously described as being type III-secreted in rhizobia, NopM and NopD.
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