Modularized CRISPR/dCas9 Effector Toolkit for Target-Specific Gene Regulation
ACS Synthetic Biology2014Vol. 3(12), pp. 986–989
Citations Over TimeTop 13% of 2014 papers
Michael Agne, Ilona Blank, Alica Joana Emhardt, Christoph G. Gäbelein, Fenja Gawlas, Nadine Gillich, Patrick Gonschorek, Thomas J. Juretschke, Stefan Krämer, Natalie Louis, Anne Müller, Alina Rudorf, Lisa M. Schäfer, Manuel C. Scheidmann, Lisa J. Schmunk, Philipp Schwenk, Maximilian R. Stammnitz, Philipp Warmer, Wilfried Weber, Adrian Fischer, Beate Kaufmann, Hanna J. Wagner, Gerald Radziwill
Abstract
The ability to control mammalian genes in a synergistic mode using synthetic transcription factors is highly desirable in fields of tissue engineering, stem cell reprogramming and fundamental research. In this study, we developed a standardized toolkit utilizing an engineered CRISPR/Cas9 system that enables customizable gene regulation in mammalian cells. The RNA-guided dCas9 protein was implemented as a programmable transcriptional activator or repressor device, including targeting of endogenous loci. For facile assembly of single or multiple CRISPR RNAs, our toolkit comprises a modular RNAimer plasmid, which encodes the required noncoding RNA components.
Related Papers
- → Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease(2016)119 cited
- → Extinction of all infectious HIV in cell culture by the CRISPR-Cas12a system with only a single crRNA(2020)108 cited
- → Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays(2018)74 cited
- → Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity(2017)71 cited
- → Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9(2022)11 cited