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A DNA methylation biomarker of alcohol consumption

Molecular Psychiatry2016Vol. 23(2), pp. 422–433

Citations Over TimeTop 1% of 2016 papers

C Liu, Riccardo E. Marioni, Åsa K. Hedman, Liliane Pfeiffer, Pei-Chien Tsai, Lindsay M. Reynolds, Allan C. Just, Qing Duan, Cindy G. Boer, Toshiko Tanaka, Cathy E. Elks, Stella Aslibekyan, Jennifer A. Brody, Brigitte Kühnel, Christian Herder, Lynn M. Almli, Deugi Zhi, Yaqiang Wang, Tianxiao Huan, Chen Yao, Michael Mendelson, Roby Joehanes, Liming Liang, S F Love, Weihua Guan, Sonia Shah, Allan F. McRae, Anja Kretschmer, Holger Prokisch, Konstantin Strauch, Annette Peters, Peter M. Visscher, Naomi R. Wray, Xiuqing Guo, Kerri L. Wiggins, Alicia K. Smith, Elisabeth B. Binder, Kerry J. Ressler, Marguerite R. Irvin, Devin Absher, Dena Hernández, Luigi Ferrucci, Stefania Bandinelli, Kurt Lohman, Jianqing Ding, Letizia Trevisi, Stefan Gustafsson, Johanna K. Sandling, Lisette Stolk, André G. Uitterlinden, İdil Yet, Juan Castillo‐Fernandez, Tim D. Spector, Joel Schwartz, Pantel Vokonas, Lars Lind, Y Li, Myriam Fornage, Donna K. Arnett, Nicholas J. Wareham, N Sotoodehnia, Ken K. Ong, Joyce B. J. van Meurs, Karen N. Conneely, Andrea Baccarelli, Ian J. Deary, Jordana T. Bell, Kari E. North, Yi Liu, Mélanie Waldenberger, Stephanie J. London, Erik Ingelsson, Daniel Levy

Abstract

The lack of reliable measures of alcohol intake is a major obstacle to the diagnosis and treatment of alcohol-related diseases. Epigenetic modifications such as DNA methylation may provide novel biomarkers of alcohol use. To examine this possibility, we performed an epigenome-wide association study of methylation of cytosine-phosphate-guanine dinucleotide (CpG) sites in relation to alcohol intake in 13 population-based cohorts (n_total=13 317; 54% women; mean age across cohorts 42-76 years) using whole blood (9643 European and 2423 African ancestries) or monocyte-derived DNA (588 European, 263 African and 400 Hispanic ancestry) samples. We performed meta-analysis and variable selection in whole-blood samples of people of European ancestry (n=6926) and identified 144 CpGs that provided substantial discrimination (area under the curve=0.90-0.99) for current heavy alcohol intake (⩾42 g per day in men and ⩾28 g per day in women) in four replication cohorts. The ancestry-stratified meta-analysis in whole blood identified 328 (9643 European ancestry samples) and 165 (2423 African ancestry samples) alcohol-related CpGs at Bonferroni-adjusted P-7. Analysis of the monocyte-derived DNA (n=1251) identified 62 alcohol-related CpGs at P-7. In whole-blood samples of people of European ancestry, we detected differential methylation in two neurotransmitter receptor genes, the γ-Aminobutyric acid-A receptor delta and γ-aminobutyric acid B receptor subunit 1; their differential methylation was associated with expression levels of a number of genes involved in immune function. In conclusion, we have identified a robust alcohol-related DNA methylation signature and shown the potential utility of DNA methylation as a clinically useful diagnostic test to detect current heavy alcohol consumption.

Citations Over TimeTop 1% of 2016 papers

Abstract

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