Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair
Citations Over TimeTop 10% of 2011 papers
Abstract
Eukaryotic cells possess a universal repair machinery that ensures rapid resealing of plasma membrane disruptions. Before resealing, the torn membrane is submitted to considerable tension, which functions to expand the disruption. Here we show that annexin-A5 (AnxA5), a protein that self-assembles into two-dimensional (2D) arrays on membranes upon Ca(2+) activation, promotes membrane repair. Compared with wild-type mouse perivascular cells, AnxA5-null cells exhibit a severe membrane repair defect. Membrane repair in AnxA5-null cells is rescued by addition of AnxA5, which binds exclusively to disrupted membrane areas. In contrast, an AnxA5 mutant that lacks the ability of forming 2D arrays is unable to promote membrane repair. We propose that AnxA5 participates in a previously unrecognized step of the membrane repair process: triggered by the local influx of Ca(2+), AnxA5 proteins bind to torn membrane edges and form a 2D array, which prevents wound expansion and promotes membrane resealing.
Related Papers
- → Evidence for the Involvement of Annexin 6 in the Trafficking between the Endocytic Compartment and Lysosomes(2001)52 cited
- → Externalization of endogenous annexin A5 participates in apoptosis of rat cardiomyocytes(2004)38 cited
- → RETRACTED: Annexin A7 modulates BAG4 and BAG4-binding proteins in mitochondrial apoptosis(2015)22 cited
- → Annexin V and Annexin VI in Rat Tissues during Postnatal Development: Immunochemical Measurements(1993)22 cited
- [Annexin A5 inhibits homocysteine-induced tissue factor expression and activity in vascular smooth muscle cells].(2009)