Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells
Citations Over TimeTop 1% of 2011 papers
Abstract
The immune synapse is an exquisitely evolved means of communication between T cells and antigen-presenting cells (APCs) during antigen recognition. Recent evidence points to the transfer of RNA via exosomes as a novel mode of intercellular communication. Here we show that exosomes of T, B and dendritic immune cells contain microRNA (miRNA) repertoires that differ from those of their parent cells. We investigate whether miRNAs are exchanged during cognate immune interactions, and demonstrate the existence of antigen-driven unidirectional transfer of miRNAs from the T cell to the APC, mediated by the delivery of CD63+ exosomes on immune synapse formation. Inhibition of exosome production by targeting neutral sphingomyelinase-2 impairs transfer of miRNAs to APCs. Moreover, miRNAs transferred during immune synapsis are able to modulate gene expression in recipient cells. Thus, our results support a mechanism of cellular communication involving antigen-dependent, unidirectional intercellular transfer of miRNAs by exosomes during immune synapsis.
Related Papers
- → Induction of heat shock proteins in B-cell exosomes(2005)446 cited
- → Current Strategies for Promoting the Large-scale Production ofExosomes(2023)55 cited
- → Unraveling the physiological functions of exosome secretion by tumors(2013)43 cited
- → Exosome subpopulations: The isolation and the functions in diseases(2023)19 cited
- → Role of Exosomes in Tuberculosis: Looking towards a Future Road Map(2023)1 cited