Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping
Nature Communications2017Vol. 8(1), pp. 15993–15993
Citations Over TimeTop 1% of 2017 papers
Stefanie L. Morgan, Natasha C. Mariano, Abel Bermudez, Nicole L. Arruda, Fangting Wu, Yunhai Luo, Gautam Shankar, Lin Jia, Huiling Chen, Ji‐Fan Hu, Andrew R. Hoffman, Chiao-Chain Huang, Sharon J. Pitteri, Ke Wang
Abstract
Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.
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