The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage

Citations Over TimeTop 10% of 2015 papers

Abstract

The Mus81 endonuclease resolves recombination intermediates and mediates cellular responses to exogenous replicative stress. Here, we show that Mus81 also regulates the rate of DNA replication during normal growth by promoting replication fork progression while reducing the frequency of replication initiation events. In the absence of Mus81 endonuclease activity, DNA synthesis is slowed and replication initiation events are more frequent. In addition, Mus81-deficient cells fail to recover from exposure to low doses of replication inhibitors and cell viability is dependent on the XPF endonuclease. Despite an increase in replication initiation frequency, cells lacking Mus81 use the same pool of replication origins as Mus81-expressing cells. Therefore, decelerated DNA replication in Mus81-deficient cells does not initiate from cryptic or latent origins not used during normal growth. These results indicate that Mus81 plays a key role in determining the rate of DNA replication without activating a novel group of replication origins.

Related Papers

• → DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p(2000)191 cited
• → TORC1 signaling regulates DNA replication via DNA replication protein levels(2018)9 cited
• → Requirement of Replication Licensing for the Dyad Symmetry Element-Dependent Replication of the Epstein–Barr Virus oriP Minichromosome(1999)23 cited
• → Replication of SV40 in vitro using proteins derived from a human cell extract(1989)10 cited
• → DNA Replication and the Cell Cycle(2007)30 cited