CD24 tracks divergent pluripotent states in mouse and human cells

Citations Over TimeTop 10% of 2015 papers

Abstract

Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

Related Papers

• → Purification of functional reprogramming factors in mammalian cell using FLAG -Tag(2017)7 cited
• → Generation of Induced Pluripotent Stem Cells(2014)16 cited
• → [Reprogramming mechanism and genetic stability of induced pluripotent stem cells (iPSCs)].(2014)1 cited
• → Progress in the reprogramming of induced pluripotent stem cells with small molecules(2015)
• → Research progress cellular reprogramming technology and induced pluripotent stem cells for retinal diseases treatment(2018)