Chromatin preparation and ChIP from Drosophila brain and discs tissues
Protocol Exchange2011
Citations Over Time
Abstract
Chromatin preparation and chromatin immunoprecipitation \(ChIP) protocol as described in Oktaba et al., 2008, Dev Cell, 15, 877-89. The protocol includes description of chromatin preparation from larval tissues, ChIP and quantitative analysis of ChIP material. Procedure 1. First "fast" dissection: Dissect for 20 minutes third instar larvae in ice-cold PBS and remove gut and fat body but leave carcass attached \(allows easier handling). Dependingon dissection speed this results in 100 to 200 carcasses per dissection round. For wild type samples we used the y strains: Canton S or w118.
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