Obtaining targeted metagenomes from uncultivated environmental Synechococcus using flow cytometry-based viral tagging

Abstract

Ocean viruses are abundant, ubiquitous, and play important roles in global biogeochemical cycles through mortality, horizontal gene transfer and manipulation of host metabolism. However, the ability to link viruses to their hosts in a high-throughput manner bottlenecks our ability to understand virus-host interactions in complex communities. Here, we present viral tagging \(VT), a method that combines mixtures of isotope labeled host cells and uorescent viruses with ow cytometry. In a single experiment, we can screen 10 7 uncultivated ocean viruses with a single strain of _Synechococcus_. These viruses can then be sequenced to quantitatively link objectively de ned environmental viral populations, and their genomes, to their hosts.

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