Whole organ and organism tissue clearing by uDISCO
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Abstract
As a novel organic solvent-based tissue clearing method, the uDISCO technique maintains the feature of tissue shrinkage while overcomes the fast signal quenching and preserves the endogenous uorescence over months. By rendering the intact organs and rodent bodies transparent while reducing their size up to 65%, uDISCO enables the light-sheet microscopy of entire body of adult mice with subcellular resolution for the rst time. Additionally, uDISCO is compatible with virus tracing, antibody labeling and over-xed human tissue staining allowing the usage in various biomedical applications. Here, we provide a detailed protocol of uDISCO describing the preparation of samples and solutions, procedure of passive clearing and whole-body clearing with perfusion system. The whole procedure takes from 2 days to 1 week depending on the tissue size.
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