A novel synthesis and detection method for cap-associated adenosine modifications in mouse mRNA
Citations Over Time
Abstract
A method is described for the detection of certain nucleotide modifications adjacent to the 5' 7-methyl guanosine cap of mRNAs from individual genes. The method quantitatively measures the relative abundance of 2'-O-methyl and N(6),2'-O-dimethyladenosine, two of the most common modifications. In order to identify and quantitatify the amounts of N(6),2'-O-dimethyladenosine, a novel method for the synthesis of modified adenosine phosphoramidites was developed. This method is a one step synthesis and the product can directly be used for the production of N(6),2'-O-dimethyladenosine containing RNA oligonucleotides. The nature of the cap-adjacent nucleotides were shown to be characteristic for mRNAs from individual genes transcribed in liver and testis.
Related Papers
- → Effects of Adenosine on Levels of Adenosine Cyclic 3',5'-Monophosphate in Human Blood Platelets in Relation to Adenosine Incorporation and Platelet Aggregation(1975)169 cited
- → Reactive and exercise hyperemia during high levels of adenosine infusion(1982)30 cited
- → Elevation of guanosine 3′,5′-monophosphate level by adenosine in cerebellar slices of guinea pig(1977)19 cited
- → Myocardial protection: The adenosine story(1996)13 cited
- → Adenosine and adenosine analogues increase blood flow in oral mucosa(1984)5 cited