A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
Scientific Reports2013Vol. 3(1), pp. 2052–2052
Citations Over TimeTop 10% of 2013 papers
Sabrina Wend, Cristina Dal Bosco, Michael M. Kämpf, Fugang Ren, Klaus Palme, Wilfried Weber, Alexander Dovzhenko, Matías D. Zurbriggen
Abstract
Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport, and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development.
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