Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system
Scientific Reports2014Vol. 4(1), pp. 3943–3943
Citations Over TimeTop 10% of 2014 papers
Yicheng Zhao, Zhen Dai, Liang Huai Yang, Minghao Yin, Kuiying Ma, Mei He, Hongsheng Ouyang, Chun‐Bo Teng
Abstract
Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells.
Related Papers
- → CRISPR/Cas9-Based Genome Editing for Disease Modeling and Therapy: Challenges and Opportunities for Nonviral Delivery(2017)550 cited
- → Cell-specific CRISPR–Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins(2019)114 cited
- → CRISPR/Cas9 Editing of the <em>C. elegans rbm-3.2</em> Gene using the <em>dpy-10</em> Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.(2020)7 cited
- → Generation of Genome-Edited Mice by Cytoplasmic Injection of CRISPR-Cas9 RNA(2023)2 cited
- → CRISPR/Cas9 Editing of the <em>C. elegans rbm-3.2</em> Gene using the <em>dpy-10</em> Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.(2020)