A Selective Glutathione Probe based on AIE Fluorogen and its Application in Enzymatic Activity Assay
Scientific Reports2014Vol. 4(1), pp. 4272–4272
Citations Over TimeTop 10% of 2014 papers
Xiaoding Lou, Yuning Hong, Sijie Chen, Chris Wai Tung Leung, Na Zhao, Bo Situ, Jacky W. Y. Lam, Ben Zhong Tang
Abstract
In this work, we design and synthesize a malonitrile-functionalized TPE derivative (TPE-DCV), which can react with thiol group through thiol-ene click reaction, leading to the fluorescence change of the system. Combined with the unique AIE property, TPE-DCV can selectively detect glutathione (GSH) but not cysteine or homocysteine. As the cleavage of GSSG with the aid of glutathione reductase produces GSH, which turns on the fluorescence of TPE-DCV, the ensemble of TPE-DCV and GSSG can thus serve as a label-free sensor for enzymatic activity assay of glutathione reductase. We also apply TPE-DCV for the detection of intracellular GSH in living cells.
Related Papers
- → Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine(1980)4,546 cited
- → Glutathione levels and activities of glutathione metabolism enzymes in patients with schizophrenia: A systematic review and meta-analysis(2019)98 cited
- → Impairment of glutathione metabolism in erythrocytes from patients with diabetes mellitus(1989)249 cited
- → In vitro inhibition of glutathione reductase by arsenotriglutathione(1995)64 cited
- → Inhibition of glutathione disulfide reductase by glutathione(1991)47 cited