Engineering an in vitro air-blood barrier by 3D bioprinting
Citations Over TimeTop 1% of 2015 papers
Abstract
Intensive efforts in recent years to develop and commercialize in vitro alternatives in the field of risk assessment have yielded new promising two- and three dimensional (3D) cell culture models. Nevertheless, a realistic 3D in vitro alveolar model is not available yet. Here we report on the biofabrication of the human air-blood tissue barrier analogue composed of an endothelial cell, basement membrane and epithelial cell layer by using a bioprinting technology. In contrary to the manual method, we demonstrate that this technique enables automatized and reproducible creation of thinner and more homogeneous cell layers, which is required for an optimal air-blood tissue barrier. This bioprinting platform will offer an excellent tool to engineer an advanced 3D lung model for high-throughput screening for safety assessment and drug efficacy testing.
Related Papers
- → Expanding Embedded 3D Bioprinting Capability for Engineering Complex Organs with Freeform Vascular Networks(2023)145 cited
- → Three-dimensional bioprinting and tissue fabrication: prospects for drug discovery and regenerative medicine(2015)36 cited
- → The Art of Building Living Tissues: Exploring the Frontiers of Biofabrication with 3D Bioprinting(2023)11 cited
- → 3D Bioprinting: A Short Overview and Future Prospects in Healthcare Engineering(2022)
- → Novel alternatives to conventional in-vitro-cancer models using 3D-bioprinting technology(2023)