Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA
Scientific Reports2015Vol. 5(1), pp. 8256–8256
Citations Over TimeTop 10% of 2015 papers
Yong Wang, Yinan Du, Bin Shen, Xiaoyang Zhou, Jian Li, Yu Liu, Jian‐Ying Wang, Jiankui Zhou, Bian Hu, Nannan Kang, Jimin Gao, Liqing Yu, Xingxu Huang, Hong Wei
Abstract
Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we achieved precise Npc1l1 targeting in Chinese Bama miniature pigs at the efficiency as high as 100%. Meanwhile, we carefully analyzed the Npc1l1 sgRNA:Cas9-mediated on- and off-target mutations in various somatic tissues and ovaries, and demonstrated that injection of zygotes with Cas9 mRNA and sgRNA is an efficient and reliable approach for generation of gene-modified pigs.
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