In Vitro Epithelial Organoid Generation Induced by Substrate Nanotopography
Citations Over TimeTop 14% of 2015 papers
Abstract
The extracellular matrix (ECM) exhibits tissue-specific topography and composition and plays a crucial role in initiating the biochemical and biomechanical signaling required for organizing cells into distinct tissues during development. How single cells assemble into structures featuring specific shapes in response to external cues is poorly understood. We examined the effect of substrate nanotopography on the morphogenesis of several types of epithelial cells and found that in response to the topography, Calu-3 and MDCK-II cells formed organoids that closely resemble their morphology in vivo. This finding represents the first demonstration that substrate nanotopography, one of the first physical cues detected by cells, can by itself induce epithelial tissue-like organization. Our results provide insights, in terms of a new aspect of ECM topography, into the design of future tissue-engineering systems and the study of mechanosignaling in the epithelium during normal development and tumor progression.
Related Papers
- → Tuning Chemistry and Topography of Nanoengineered Surfaces to Manipulate Immune Response for Bone Regeneration Applications(2017)292 cited
- → Small surface nanotopography encourages fibroblast and osteoblast cell adhesion(2013)70 cited
- → Stem cell responses to nanotopography(2014)46 cited
- → Nanotopographic features induced by plasma flame modulate the osteoinductivity of titanium alloy(2022)1 cited
- → A Systematic Study of Cell Mechanics and Function Modulated by Nanotopography(2019)