Fluorescence-Raman Dual Modal Endoscopic System for Multiplexed Molecular Diagnostics
Citations Over TimeTop 10% of 2015 papers
Abstract
Optical endoscopic imaging, which was recently equipped with bioluminescence, fluorescence, and Raman scattering, allows minimally invasive real-time detection of pathologies on the surface of hollow organs. To characterize pathologic lesions in a multiplexed way, we developed a dual modal fluorescence-Raman endomicroscopic system (FRES), which used fluorescence and surface-enhanced Raman scattering nanoprobes (F-SERS dots). Real-time, in vivo, and multiple target detection of a specific cancer was successful, based on the fast imaging capability of fluorescence signals and the multiplex capability of simultaneously detected SERS signals using an optical fiber bundle for intraoperative endoscopic system. Human epidermal growth factor receptor 2 (HER2) and epidermal growth factor receptor (EGFR) on the breast cancer xenografts in a mouse orthotopic model were successfully detected in a multiplexed way, illustrating the potential of FRES as a molecular diagnostic instrument that enables real-time tumor characterization of receptors during routine endoscopic procedures.
Related Papers
- → Developing a Bright NIR‐II Fluorophore with Fast Renal Excretion and Its Application in Molecular Imaging of Immune Checkpoint PD‐L1(2018)116 cited
- → Fluorescence lifetime imaging microscopy in the medical sciences(2014)55 cited
- → Principle and Application of Fluorescence Lifetime Imaging for Neuroscience(2019)14 cited
- → Cover Feature: Responsive Fluorophore Aggregation Provides Spectral Contrast for Fluorescence Lifetime Imaging (ChemBioChem 15/2020)(2020)1 cited
- [Fluorescence lifetime imaging microscopy (FLIM) in biological and medical research].(2009)