Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
Scientific Reports2015Vol. 5(1), pp. 10734–10734
Citations Over TimeTop 10% of 2015 papers
Melissa A. Graewert, Daniel Franke, Cy M. Jeffries, Clément Blanchet, Darja Ruskule, Katja Kuhle, Antje Flieger, Bernd Schäfer, Bernd Tartsch, Rob Meijers, Dmitri I. Svergun
Abstract
Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).
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