A cost-effective and efficient reprogramming platform for large-scale production of integration-free human induced pluripotent stem cells in chemically defined culture
Citations Over TimeTop 10% of 2015 papers
Abstract
Factors limiting the adoption of iPSC technology include the cost of developing lines and the time period that it takes to characterize and bank them, particularly when integration free, feeder free, and Xeno-free components are used. In this manuscript we describe our optimization procedure that enables a single technician to make 20-40 lines at a time in a 24-96 well format in a reliable and reproducible fashion. Improvements spanned the entire workflow and included using RNA virus, reducing cytotoxicity of reagents, developing improved transfection and freezing efficiencies, modifying the manual colony picking steps, enhancing passaging efficiency and developing early criteria of success. These modifications allowed us to make more than two hundred well-characterized lines per year.
Related Papers
- → Purification of functional reprogramming factors in mammalian cell using FLAG -Tag(2017)7 cited
- → Generation of Induced Pluripotent Stem Cells(2014)16 cited
- → Generation of Induced Pluripotent Stem Cells(2020)1 cited
- → [Reprogramming mechanism and genetic stability of induced pluripotent stem cells (iPSCs)].(2014)1 cited
- → Progress in the reprogramming of induced pluripotent stem cells with small molecules(2015)