Cell-free expression of functional receptor tyrosine kinases
Scientific Reports2015Vol. 5(1), pp. 12896–12896
Citations Over TimeTop 10% of 2015 papers
Wei He, Tiffany M. Scharadin, Matthew Saldana, Candice Gellner, Steven Hoang-Phou, Christina L. Takanishi, Greg L. Hura, John A. Tainer, Kermit L. Carraway, Paul T. Henderson, Matthew A. Coleman
Abstract
Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes, and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I based nanolipoprotein particles.
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