High-yield isolation of extracellular vesicles using aqueous two-phase system
Scientific Reports2015Vol. 5(1), pp. 13103–13103
Citations Over TimeTop 10% of 2015 papers
Hyun‐Woo Shin, Chungmin Han, Joseph M. Labuz, Jiyoon Kim, Jongmin Kim, Siwoo Cho, Yong Song Gho, Shuichi Takayama, Jaesung Park
Abstract
Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (~70%) in a short time (~15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.
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