A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease
Scientific Reports2015Vol. 5(1), pp. 14440–14440
Citations Over TimeTop 19% of 2015 papers
Brian L. Gilmore, Carly E. Winton, Andrew C. Demmert, Justin R. Tanner, Sam Bowman, Vasilea Karageorge, Kaya Patel, Zhi Sheng, Deborah F. Kelly
Abstract
We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.
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