Cep55 regulates spindle organization and cell cycle progression in meiotic oocyte
Citations Over TimeTop 15% of 2015 papers
Abstract
Cep55 is a relatively novel member of the centrosomal protein family. Here, we show that Cep55 is expressed in mouse oocytes from the germinal vesicle (GV) to metaphase II (MII) stages. Immuostaining and confocal microscopy as well as time lapse live imaging after injection of mRNA encoding fusion protein of Cep55 and GFP identified that Cep55 was localized to the meiotic spindle, especially to the spindle poles at metaphase, while it was concentrated at the midbody in telophase in meiotic oocytes. Knockdown of Cep55 by specific siRNA injection caused the dissociation of γ-tubulin from the spindle poles, resulting in severely defective spindles and misaligned chromosomes, leading to metaphase I arrest and failure of first polar body (PB1) extrusion. Correspondingly, cyclin B accumulation and spindle assembly checkpoint (SAC) activation were observed in Cep55 knockdown oocytes. Our results suggest that Cep55 may act as an MTOC-associated protein regulating spindle organization, and thus cell cycle progression during mouse oocyte meiotic maturation.
Related Papers
- → WD40-repeat protein 62 is a JNK-phosphorylated spindle pole protein required for spindle maintenance and timely mitotic progression.(2012)81 cited
- → CDCA8 regulates meiotic spindle assembly and chromosome segregation during human oocyte meiosis(2020)27 cited
- → Donor Centrosome Regulation of Initial Spindle Formation in Mouse Somatic Cell Nuclear Transfer: Roles of Gamma-Tubulin and Nuclear Mitotic Apparatus Protein 11(2006)35 cited
- → Bora regulates meiotic spindle assembly and cell cycle during mouse oocyte meiosis(2013)9 cited
- Centrosomal Protein192(Cep192) is Essential for Formation of Spindle Assembly and Microtubule during Porcine Oocyte Maturation(2017)