The CagA toxin of Helicobacter pylori: abundant production but relatively low amount translocated

Citations Over TimeTop 10% of 2016 papers

Abstract

CagA is one of the most studied pathogenicity factors of the bacterial pathogen Helicobacter pylori. It is injected into host cells via the H. pylori cag-Type IV secretion system. Due to its association with gastric cancer, CagA is classified as oncogenic protein. At the same time CagA represents the 4(th) most abundant protein produced by H. pylori, suggesting that high amounts of toxin are required to cause the physiological changes or damage observed in cells. We were able to quantify the injection of CagA into gastric AGS epithelial cells in vitro by the adaptation of a novel protease-based approach to remove the tightly adherent extracellular bacteria. After one hour of infection only 1.5% of the total CagA available was injected by the adherent bacteria, whereas after 3 hours 7.5% was found within the host cell. Thus, our data show that only a surprisingly small amount of the CagA available in the infection is finally injected under in vitro infection conditions.

Related Papers

• → babA2- and cagA -Positive Helicobacter pylori Strains Are Associated with Duodenal Ulcer and Gastric Carcinoma in Brazil(2003)78 cited
• → Оцінка якості життя пацієнтів із пептичною виразкою шлунка та дванадцятипалої кишки у поєднанні з артеріальною гіпертензією та цукровим діабетом 2-го типу за наявності токсигенних (cagA+, vacA+) штамів Helicobacter pylori(2020)2 cited
• [High prevalence of VacA, CagA protein expression and presence of cag pathogenicity island in Japanese Helicobacter pylori isolates].(1999)
• → Клинические проявления и эндоскопически-гистологические изменения у детей с хроническим гастритом, индуцированным Helicobacter pylori, с разной токсигенностью(2019)
• → PREVALENCE OF CAGA GENE AND VACA GENOTYPES OF HELICOBACTER PYLORI AMONG PATIENTS WITH GASTRODUODENAL DISSEASES(2022)