Monoclonal regulatory T cells provide insights into T cell suppression

Citations Over Time

Abstract

Regulatory T cells (Tregs) have a crucial role in maintaining lymphocyte homeostasis. However an understanding of how Tregs function at a cellular and molecular level has not yet been fully elucidated. Here, we make use of a T cell receptor (TCR) transgenic, Rag(-/-) mouse expressing a Forkhead-Box-Protein P3 (Foxp3) transgene. This mouse provides a source of monoclonal CD4(+) Foxp3(+) T cells with a defined specificity. Here we show that monoclonal B3K506 Tregs are functional in vitro and in vivo and clearly require cognate antigen to be suppressive. We further show that the strength of Treg stimulation determines the strength of Treg mediated suppression. Finally we analysed various suppressive mechanisms used by monoclonal Tregs and found that Treg-Tconv proximity is a parameter, which correlates with enhanced suppression.

Related Papers

• → SIRT1 transgenic mice show phenotypes resembling calorie restriction(2007)708 cited
• → TCR transgenes and transgene cassettes for TCR gene therapy: status in 2008(2009)61 cited
• → Transgenic mice: beyond the knockout(2010)24 cited
• → Early Changes in Behavior Deficits, Amyloid β-42 Deposits and MAPK Activation in Doubly Transgenic Mice Co-expressing NSE-Controlled Human Mutant PS2 and APPsw(2005)6 cited
• → Development and Characterization of Thy*IκBα-SI Transgenic Mice(2010)1 cited