Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry
Scientific Reports2016Vol. 6(1), pp. 28107–28107
Citations Over TimeTop 18% of 2016 papers
André C. Müller, Roberto Giambruno, Juliane Weißer, Peter Májek, Alexandre Hofer, Johannes W. Bigenzahn, Giulio Superti‐Furga, Henning J. Jessen, Keiryn L. Bennett
Abstract
Mass spectrometry-based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically-altered enzyme-cofactor systems. We describe a method that combines stable γ-[(18)O2]-ATP with classical in vitro kinase assays within a contemporary quantitative proteomic workflow. Our approach improved detection of known substrates of the non-receptor tyrosine kinase ABL1; and identified potential, new in vitro substrates.
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