Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
Scientific Reports2016Vol. 6(1), pp. 28209–28209
Citations Over TimeTop 1% of 2016 papers
Marzena Stefaniuk, Emilio J. Gualda, Monika Pawłowska, Diana Legutko, Paweł Matryba, Paulina Koza, Witold Konopka, Dorota Owczarek, Marcin Wawrzyniak, Pablo Loza‐Álvarez, Leszek Kaczmarek
Abstract
Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a similar result for rats has proven difficult to achieve. Herein, we report on creating novel transgenic rat harboring fluorescent reporter GFP under control of neuronal gene promoter. We then present data on clearing the rat brain, showing that FluoClearBABB was found superior over passive CLARITY and CUBIC methods. Finally, we demonstrate efficient imaging of the rat brain using light-sheet fluorescence microscopy.
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