An ABA-increased interaction of the PYL6 ABA receptor with MYC2 Transcription Factor: A putative link of ABA and JA signaling
Citations Over TimeTop 1% of 2016 papers
Abstract
Abscisic acid (ABA) is a plant hormone that mediates abiotic stress tolerance and regulates growth and development. ABA binds to members of the PYL/RCAR ABA receptor family that initiate signal transduction inhibiting type 2C protein phosphatases. Although crosstalk between ABA and the hormone Jasmonic Acid (JA) has been shown, the molecular entities that mediate this interaction have yet to be fully elucidated. We report a link between ABA and JA signaling through a direct interaction of the ABA receptor PYL6 (RCAR9) with the basic helix-loop-helix transcription factor MYC2. PYL6 and MYC2 interact in yeast two hybrid assays and the interaction is enhanced in the presence of ABA. PYL6 and MYC2 interact in planta based on bimolecular fluorescence complementation and co-immunoprecipitation of the proteins. Furthermore, PYL6 was able to modify transcription driven by MYC2 using JAZ6 and JAZ8 DNA promoter elements in yeast one hybrid assays. Finally, pyl6 T-DNA mutant plants show an increased sensitivity to the addition of JA along with ABA in cotyledon expansion experiments. Overall, the present study identifies a direct mechanism for transcriptional modulation mediated by an ABA receptor different from the core ABA signaling pathway, and a putative mechanistic link connecting ABA and JA signaling pathways.
Related Papers
- → A complex signaling trio in seed germination: Auxin-JA-ABA(2023)30 cited
- Role of Jasmonic Acid and Abscisic Acid Treatments in Alleviating the Adverse Effects of Drought Stress and Regulating Trypsin Inhibitor Production in Soybean Plant.(2009)
- → Effect of jasmonic acid on endogenous gibberellins and abscisic acid in rice under NaCl stress(2005)29 cited
- → Interaction of abscisic acid and jasmonic acid on the inhibition of seed germination and the induction of freezing tolerance(1994)29 cited
- → Members of the abscisic acid co-receptor PP2C protein family mediate salicylic acid-abscisic acid crosstalk(2017)4 cited