Capturing tumor complexity in vitro: Comparative analysis of 2D and 3D tumor models for drug discovery
Scientific Reports2016Vol. 6(1), pp. 28951–28951
Citations Over TimeTop 10% of 2016 papers
Kristin Stock, Marta F Estrada, Suzana Vidic, Kjersti Gjerde, Albin Rudisch, Vítor Espirito Santo, Michaël Barbier, Sami Blom, Sharath C. Arundkar, Irwin R. Selvam, Annika Oßwald, Yan Stein, Sylvia Gruenewald, Catarina Brito, Wytske M. van Weerden, Varda Rotter, Erwin R. Boghaert, Moshe Oren, Wolfgang Sommergruber, Yolanda Chong, Ronald De Hoogt, Ralph Graeser
Abstract
Two-dimensional (2D) cell cultures growing on plastic do not recapitulate the three dimensional (3D) architecture and complexity of human tumors. More representative models are required for drug discovery and validation. Here, 2D culture and 3D mono- and stromal co-culture models of increasing complexity have been established and cross-comparisons made using three standard cell carcinoma lines: MCF7, LNCaP, NCI-H1437. Fluorescence-based growth curves, 3D image analysis, immunohistochemistry and treatment responses showed that end points differed according to cell type, stromal co-culture and culture format. The adaptable methodologies described here should guide the choice of appropriate simple and complex in vitro models.
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