CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing
Scientific Reports2016Vol. 6(1), pp. 28973–28973
Citations Over TimeTop 10% of 2016 papers
Abstract
The advent of next generation gene editing technologies has revolutionized the fields of genome engineering in allowing the generation of gene knockout models and functional gene analysis. However, the screening of resultant clones remains challenging due to the simultaneous presence of different indels. Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping up to three alleles from a single Sanger sequencing trace, providing a robust and readily accessible platform to directly identify indels and significantly speed up the characterization of clones.
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