Circulating tumor DNA detection in lung cancer patients before and after surgery

Citations Over TimeTop 10% of 2016 papers

Abstract

Circulating tumor DNA (ctDNA) in peripheral blood is a "liquid biopsy" that contains representative tumor information including gene mutations. Additionally, repeated ctDNA samples can be easily obtained to monitor response to treatment and disease progression, which may be especially valuable to lung cancer patients with tumors that cannot be easily biopsied or removed. To investigate the changes in ctDNA after surgical tumor resection, tumor and blood samples obtained before and after surgery were collected prospectively from 41 non-small lung cancer (NSCLC) patients. Somatic driver mutations in tumor DNA (tDNA) and pre- and post-op plasma ctDNA sample pairs were identified by targeted sequencing in several genes including EGFR, KRAS, and TP53 with an overall study concordance of 78.1% and sensitivity and specificity of 69.2% and 93.3%, respectively. Importantly, the frequency of 91.7% of ctDNA mutations decreased after surgery and these changes were observed as little as 2 days post-op. Moreover, the presence of ctDNA had a higher positive predictive value than that of six tumor biomarkers in current clinical use. This study demonstrates the use of targeted sequencing to reliably identify ctDNA changes in response to treatment, indicating a potential utility of this approach in the clinical management of NSCLC.

Related Papers

• → Current Status of CTCs as Liquid Biopsy in Lung Cancer and Future Directions(2015)71 cited
• → Combination of microfluidic chips and biosensing for the enrichment of circulating tumor cells(2022)30 cited
• → The rationale for liquid biopsy in colorectal cancer: a focus on circulating tumor cells(2015)20 cited
• → Exploration of Circulating Tumour Cell (CTC) Biology: A Paradigm Shift in Liquid Biopsy(2020)19 cited
• → Clinical Applications of Circulating Tumor Cells in Pharmacotherapy: Challenges and Perspectives(2017)16 cited