MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection

Citations Over TimeTop 13% of 2016 papers

Abstract

Neuroregeneration and apoptosis are two important pathophysiologic changes after spinal cord injury (SCI), but their underlying mechanisms remain unclear. MicroRNAs (miRNAs) play a crucial role in the regulation of neuroregeneration and neuronal apoptosis, research areas that have been greatly expanded in recent years. Here, using miRNA arrays to profile miRNA transcriptomes, we demonstrated that miR-127-3p was significantly down-regulated after spinal cord transection (SCT). Then, bioinformatics analyses and experimental detection showed that miR-127-3p exhibited specific effects on the regulation of neurite outgrowth and the induction of neuronal apoptosis by regulating the expression of the mitochondrial membrane protein mitoNEET. Moreover, knockdown of MitoNEET leaded to neuronal loss and apoptosis in primary cultured spinal neurons. This study therefore revealed that miR-127-3p, which targets mitoNEET, plays a vital role in regulating neurite outgrowth and neuronal apoptosis after SCT. Thus, modificatioin of the mitoNEET expression, such as mitoNEET activition may provide a new strategy for the treatment of SCI in preclinical trials.

Related Papers

• → The Neuroprotective Functions of Transforming Growth Factor Beta Proteins(2012)252 cited
• → Delving into the recent advancements of spinal cord injury treatment: a review of recent progress(2021)68 cited
• → The Role of Bioactive Compounds on the Promotion of Neurite Outgrowth(2012)85 cited
• → Molecular mechanisms of estrogen for neuroprotection in spinal cord injury and traumatic brain injury(2015)65 cited
• → New Strategies in Neuroprotection and Neurorepair(2011)17 cited