A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles
Citations Over TimeTop 10% of 2016 papers
Abstract
A novel detection method of small molecules, competitive bio-barcode amplification immunoassay, was developed and described in this report. Through the gold nanoparticles (AuNPs) probe and magnetic nanoparticles (MNPs) probe we prepared, only one monoclonal antibody can be used to detect small molecules. The competitive bio-barcode amplification immunoassay overcomes the obstacle that the bio-barcode assay cannot be used in small molecular detection, as two antibodies are unable to combine to one small molecule due to its small molecular structure. The small molecular compounds, triazophos, were selected as targets for the competitive bio-barcode amplification immunoassay. The linear range of detection was from 0.04 ng mL-1 to 10 ng mL-1, and the limit of detection (LOD) was 0.02 ng mL-1, which was 10-20 folds lower than ELISA (Enzyme Linked Immunosorbent Assay). A practical application of the proposed immunoassay was evaluated by detecting triazophos in real samples. The recovery rate ranged from 72.5% to 110.5%, and the RSD was less than 20%. These results were validated by GC-MS, which indicated that this convenient and sensitive method has great potential for small molecular in real samples.
Related Papers
- → Rapid Determination of Fumonisin B1 in Food Samples by Enzyme-Linked Immunosorbent Assay and Colloidal Gold Immunoassay(2006)144 cited
- → Thionine/nanogold multilayer film for electrochemical immunoassay of alpha-fetoprotein in human serum using biofunctional double-codified gold nanoparticles(2010)34 cited
- → A sensitive immunosensor based on FRET between gold nanoparticles and InP/ZnS quantum dots for arginine kinase detection(2021)27 cited
- → Homogeneous immunoassay based on aggregation of antibody-functionalized gold nanoparticles coupled with light scattering detection(2008)53 cited
- → A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples(2008)32 cited