Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
Scientific Reports2017Vol. 7(1), pp. 39846–39846
Citations Over TimeTop 10% of 2017 papers
Georgia K. Atkin‐Smith, Stephanie Paone, Damien Zanker, Mubing Duan, Thanh Kha Phan, Weisan Chen, Mark D. Hulett, Ivan K. H. Poon
Abstract
Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.
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