Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics
Scientific Reports2017Vol. 7(1), pp. 39955–39955
Citations Over TimeTop 10% of 2017 papers
Shutao Wang, Tara Kugelman, A. Buch, Mathieu Herman, Yang Han, Maria Eleni Karakatsani, S. Abid Hussaini, Karen Duff, Elisa E. Konofagou
Abstract
Optogenetics, a widely used technique in neuroscience research, is often limited by its invasive nature of application. Here, we present a noninvasive, ultrasound-based technique to introduce optogenetic channels into the brain by temporarily opening the blood-brain barrier (BBB). We demonstrate the efficiency of the method developed and evaluate the bioactivity of the non-invasively introduced channelrhodopsin channels by performing stimulation in freely behaving mice.
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