Quantification of Detergents Complexed with Membrane Proteins
Citations Over TimeTop 10% of 2017 papers
Abstract
Most membrane proteins studies require the use of detergents, but because of the lack of a general, accurate and rapid method to quantify them, many uncertainties remain that hamper proper functional and structural data analyses. To solve this problem, we propose a method based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) that allows quantification of pure or mixed detergents in complex with membrane proteins. We validated the method with a wide variety of detergents and membrane proteins. We automated the process, thereby allowing routine quantification for a broad spectrum of usage. As a first illustration, we show how to obtain information of the amount of detergent in complex with a membrane protein, essential for liposome or nanodiscs reconstitutions. Thanks to the method, we also show how to reliably and easily estimate the detergent corona diameter and select the smallest size, critical for favoring protein-protein contacts and triggering/promoting membrane protein crystallization, and to visualize the detergent belt for Cryo-EM studies.
Related Papers
- → Performance and Cost Analysis of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Routine Identification of Yeast(2011)291 cited
- → A new combination MALDI matrix for small molecule analysis: application to imaging mass spectrometry for drugs and metabolites(2012)69 cited
- Primary research on identification of common probiotics by MALDI-TOF mass spectrometry.(2010)
- → Matrix free MALDI mass spectrometry for phytochemical investigations(2006)