Double-flow focused liquid injector for efficient serial femtosecond crystallography
Citations Over TimeTop 10% of 2017 papers
Abstract
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double-flow focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices [corrected].
Related Papers
- → Gas Injection from Slot Nozzles with Various Shapes in Water.(2000)10 cited
- → R744 multi ejector development.(2016)3 cited
- Research on Multifunctional Nozzle(2001)
- A study devoted to the design of a special nozzle with multiple functions.(2002)
- → Aeral injector for combustor of small unmanned aircraft – results of investigation(2006)